Mineral Medium with Benzylcyanide (DSMZ Medium 465d)

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Benzylcyanide
(DSMZ Medium 465d)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·2H 2 O.............................................................................3.5g
KH 2 PO 4 .........................................................................................1.0g
MgCl 2 ·6H 2 O .................................................................................0.1g
Ca(NO 3 ) 2 ·4H 2 O ..........................................................................0.05g
Benzylcyanide solution............................................................10.0mL
Glucose solution ......................................................................10.0mL
Trace elements solution SL-4....................................................1.0mL
pH 7.25 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
Na 2 MoO 4 ·H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Glucose Solution:
Composition per 10.0mL:
Glucose.........................................................................................1.8g
Preparation of Glucose Solution: Add glucose to 10.0mL dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Benzylcyanide Solution:
Composition per 100.0mL:
Benzylcyanide.............................................................................0.12g
Preparation of Benzylcyanide Solution: Add benzylcyanide to
10.0mL distilled/deionized water. Mix thoroughly. Do not sterilize.
Preparation of Medium:  Add components, except benzylcyanide
solution and glucose solution, to 980.0mL distilled/deionized water.
Adjust pH to 7.25. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Aseptically add 10.0mL glucose solution and
10.0mL benzylcyanide solution to the medium. Mix thoroughly. Asep-
tically distribute the medium to sterile tubes or flasks.
Use: For the cultivation of Pseudomonas sp., Rhodococcus erythropo-
lis, and other benzylcyanide-utilizing bacteria.