Mineral Medium, Brunner

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium, Brunner

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................2.44g
KH 2 PO 4 .......................................................................................1.52g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.05g
Trace elements solution SL-4 ..................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................. 0.2g
Trace elements solution SL-6 ................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Trace Elements Solution SL-4:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Alcaligenes species,
Bacillus benzoevorans, Bacillus gordonae, Comamonas acidovorans,
Hyphomicrobium species, Moraxella species, Nocardia species,
Pseudomonas species, Rhodococcus species, Sphingomonas species,
and Xanthobacter species.