Mineral Medium with Camphor

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Camphor

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·12H 2 O...........................................................................9.0g
Ferric ammonium citrate...............................................................5.0g
MnSO 4 ·H 2 O..................................................................................3.0g
NH 4 Cl ...........................................................................................2.0g
KH 2 PO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.2g
Titriplex I.................................................................................10.0mg
CoSO 4 .......................................................................................10.0μg
Camphor crumbs.....................................................................variable
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except camphor
crumbs, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Inoculate tubes or flasks and place in a dessicator
jar in which crumbs of camphor will be evaporated.
Use: For the cultivation of bacteria that can utilize camphor as sole
carbon source.