Mineral Medium for Chemolithotrophic Growth

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium for Chemolithotrophic Growth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 985.0mL:

Agar............................................................................................15.0g
Na 2 HPO 4 ·2H 2 O.............................................................................2.9g
KH 2 PO 4 .........................................................................................2.3g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaHCO 3 ........................................................................................0.5g
CaCl 2 ·2H 2 O ................................................................................0.01g
Ferric ammonium citrate solution............................................20.0mL
Trace elements solution SL-6....................................................5.0mL
pH 6.8 ± 0.2 at 25°C
Ferric Ammonium Citrate Solution:
Composition per 20.0mL:
Ferric ammonium citrate.............................................................0.05g
Preparation of Ferric Ammonium Citrate Solution:  Add fer-
ric ammonium citrate to distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C.
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6 : Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly.
Preparation of Medium: Add components, except ferric ammoni-
um citrate solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
add 20.0mL of sterile ferric ammonium citrate solution. Mix thorough-
ly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the chemolithotrophic growth and cultivation of a wide vari-
ety of bacteria.