Mineral Medium with Chloridazon

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Chloridazon

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Chloridazon...................................................................................1.0g
Na 2 HPO 4 ·12H 2 O...........................................................................0.7g
(NH 4 ) 2 HPO 4 ..................................................................................0.7g
KH 2 PO 4 .........................................................................................0.3g
(NH 4 )H 2 PO 4 ..................................................................................0.3g
MgSO 4 ·7H 2 O..............................................................................0.25g
(NH 4 ) 2 SO 4 ....................................................................................0.1g
CaCl 2 ·6H 2 O ................................................................................0.05g
H 3 BO 3 ........................................................................................0.5mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
FeCl 3 ·6H 2 O................................................................................0.2mg
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O.................................................................0.2mg
KI...............................................................................................0.1mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Vitamin solution.......................................................................20.0mL
pH 6.8–7.0 at 25°C
Vitamin Solution:
Composition per 20.0mL:
Biotin .........................................................................................0.1mg
Vitamin B 12 ..............................................................................0.03mg
Preparation of Vitamin Solution: Add biotin and vitamin B 12 to
20.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Adjust pH to 6.8–7.0 with 1N NaOH. Autoclave for 20 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add the sterile
vitamin solution. Mix thoroughly. Distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Phenylobacterium immo-
bile.