Mineral Medium with Crude Oil

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Crude Oil

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 100.0mL:

K 2 HPO 4 .......................................................................................0.45g
(NH 4 ) 2 SO 4 ....................................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.02g
NaCl............................................................................................0.01g
CaCl 2 ...........................................................................................0.01g
FeCl 3 .........................................................................................0.002g
Crude oil ....................................................................................5.0mL
pH 7.2 ± 0.3 at 25°C
Preparation of Medium: Add components, except crude oil, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 5.0mL of filter-sterilized crude oil. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of Acinetobacter baumannii.