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Mineral Medium with Cyanuric Acid as Nitrogen Source (DSMZ Medium 465g)
[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]
Mineral Medium
with Cyanuric Acid as Nitrogen Source
(DSMZ Medium 465g)
KH 2 PO 4 .........................................................................................1.0g
MgCl 2 ·6H 2 O .................................................................................0.1g
Ca(NO 3 ) 2 ·4H 2 O ..........................................................................0.05g
Cyanuric acid solution.............................................................10.0mL
Vitamin solution.......................................................................10.0mL
Glycerol solution .....................................................................10.0mL
Trace elements solution SL-4....................................................1.0mL
pH 7.25 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
Na 2 MoO 4 ·H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Vitamin B 12 ..............................................................................50.0mg
Pantothenic acid.......................................................................50.0mg
Riboflavin................................................................................50.0mg
Alpha-lipoic acid .....................................................................50.0mg
p-Aminobenzoic acid...............................................................50.0mg
Thiamine-HCl·2H 2 O................................................................50.0mg
Nicotinic acid...........................................................................25.0mg
Nicotinamide............................................................................25.0mg
Biotin .......................................................................................20.0mg
Folic acid..................................................................................20.0mg
Pyridoxamine-HCl...................................................................10.0mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Cyanuric Acid Solution:
Composition per 10.0mL:
Cyanuric acid.........................................................................645.0mg
Preparation of Cyanuric Acid Solution: Add cyanuric acid to
10.0mL distilled/deionized water. Mix thoroughly. Adjust pH to 7.0.
Filter sterilize.
Glycerol Solution:
Composition per 10.0mL:
Glycerol ........................................................................................5.5g
Preparation of Glycerol Solution: Add glycerol to distilled/de-
ionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except cyanuric acid so-
lution, glycerol solution, and vitamin solution, to 1.0L distilled/deion-
ized water. Adjust pH to 7.25. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL cyanuric acid solution, 10.0mL glyc-
erol solution, and 10.0mL vitamin solution. Mix thoroughly. Aseptical-
ly distribute to sterile tubes or flasks.
Use: For the cultivation of Gordonia rubripertincta=Rhodococcus
rubropertinctus and other cyanuric acid-utilizing bacteria.
with Cyanuric Acid as Nitrogen Source
(DSMZ Medium 465g)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Na 2 HPO 4 ·2H 2 O.............................................................................3.5gKH 2 PO 4 .........................................................................................1.0g
MgCl 2 ·6H 2 O .................................................................................0.1g
Ca(NO 3 ) 2 ·4H 2 O ..........................................................................0.05g
Cyanuric acid solution.............................................................10.0mL
Vitamin solution.......................................................................10.0mL
Glycerol solution .....................................................................10.0mL
Trace elements solution SL-4....................................................1.0mL
pH 7.25 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
Na 2 MoO 4 ·H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Vitamin B 12 ..............................................................................50.0mg
Pantothenic acid.......................................................................50.0mg
Riboflavin................................................................................50.0mg
Alpha-lipoic acid .....................................................................50.0mg
p-Aminobenzoic acid...............................................................50.0mg
Thiamine-HCl·2H 2 O................................................................50.0mg
Nicotinic acid...........................................................................25.0mg
Nicotinamide............................................................................25.0mg
Biotin .......................................................................................20.0mg
Folic acid..................................................................................20.0mg
Pyridoxamine-HCl...................................................................10.0mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Cyanuric Acid Solution:
Composition per 10.0mL:
Cyanuric acid.........................................................................645.0mg
Preparation of Cyanuric Acid Solution: Add cyanuric acid to
10.0mL distilled/deionized water. Mix thoroughly. Adjust pH to 7.0.
Filter sterilize.
Glycerol Solution:
Composition per 10.0mL:
Glycerol ........................................................................................5.5g
Preparation of Glycerol Solution: Add glycerol to distilled/de-
ionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except cyanuric acid so-
lution, glycerol solution, and vitamin solution, to 1.0L distilled/deion-
ized water. Adjust pH to 7.25. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL cyanuric acid solution, 10.0mL glyc-
erol solution, and 10.0mL vitamin solution. Mix thoroughly. Aseptical-
ly distribute to sterile tubes or flasks.
Use: For the cultivation of Gordonia rubripertincta=Rhodococcus
rubropertinctus and other cyanuric acid-utilizing bacteria.
