Mineral Medium with Dichlorobenzoate

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Dichlorobenzoate

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................2.78g
KH 2 PO 4 .......................................................................................2.78g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
Hutner’s mineral base..............................................................20.0mL
2,4-Dichlorobenzoate solution.................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Hutner’s Mineral Base:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O.................................................................................3.34g
FeSO 4 ·7H 2 O.............................................................................99.0mg
(NH 4 ) 2 MoO 4 ............................................................................9.25mg
Metals “44”..............................................................................50.0mL
Preparation of Hutner’s Mineral Base: Add nitrilotriacetic acid to
500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with
KOH. Add remaining components. Readjust pH to 7.2 with H 2 SO 4 or
KOH. Add distilled/deionized water to 1.0L.
Metals “44”:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
CuSO 4 ·5H 2 O...............................................................................0.04g
Co(NO 3 ) 2 ·6H 2 O ........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O........................................................................0.018g
Preparation of Metals “44”: Add a few drops of H 2 SO 4 to dis-
tilled/deionized water to inhibit precipitate formation. Add compo-
nents to acidified distilled/deionized water and bring volume to
100.0mL. Mix thoroughly.
2,4-Dichlorobenzoate Solution:
Composition per 10.0mL:
2,4-Dichlorobenzoate.................................................................5.0mg
Preparation of 2,4-Dichlorobenzoate Solution: Add 2,4-di-
chlorobenzoate to 10.0mL of distilled/deionized water. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except 2,4-dichlo-
robenzoate solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Adjust pH to 6.8 with 1N KOH. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add the sterile 2,4-dichlorobenzoate solution. Mix thoroughly. Distrib-
ute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Actinomyces viscosus.