Mineral Medium with Glucose

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Glucose

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Na 2 HPO 4 .......................................................................................4.8g
KH 2 PO 4 .........................................................................................4.4g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Solution B................................................................................10.0mL
Solution A..................................................................................5.0mL
pH 6.8 ± 0.2 at 25°C
Solution A:
Composition per 100.0mL:
Ferric ammonium citrate...............................................................1.0g
CaCl 2 .............................................................................................0.1g
Preparation of Solution A: Add ferric ammonium citrate and
CaCl 2 to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Solution B:
Composition per 100.0mL:
Glucose.......................................................................................10.0g
Preparation of Solution B: Add glucose to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except solution A and
solution B, to distilled/deionized water and bring volume to 985.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°C. Aseptically add sterile solu-
tion A and sterile solution B. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Alcaligenes latus.