Mineral Medium H-3

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium H-3

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar (if needed)..........................................................................20.0g
Na 2 HPO 4 ·2H 2 O............................................................................2.9g
KH 2 PO 4 ........................................................................................2.3g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaHCO 3 ........................................................................................0.5g
CaCl 2 ·2H 2 O................................................................................0.01g
Ferric ammonium citrate solution............................................20.0mL
Trace elements solution.............................................................5.0mL
Ferric Ammonium Citrate Solution:
Composition per 20.0mL:
Ferric ammonium citrate.............................................................0.05g
Preparation of Ferric Ammonium Citrate Solution: Add fer-
ric ammonium citrate to distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C.
Trace Elements Solution:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 3 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O...............................................................................0.03g
Na 2 MoO 4 ·2H 2 O..........................................................................0.03g
CuCl 2 ·2H 2 O................................................................................0.01g
NiCl 2 ·6H 2 O...............................................................................0.002g
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except ferric ammoni-
um citrate solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
add 20.0mL of sterile ferric ammonium citrate solution. Mix thorough-
ly. Pour into sterile Petri dishes or distribute into sterile tubes. Incubate
cultures in 60% H 2  + 28% N 2  + 10% CO 2  + 2% O 2 .
Use: For the chemolithotrophic growth of Alcaligenes eutrophus.