Mineral Medium for Hydrogen Bacteria

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium for Hydrogen Bacteria

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Na 2 HPO 4 ·2H 2 O.............................................................................2.9g
KH 2 PO 4 .........................................................................................2.3g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaHCO 3 ........................................................................................0.5g
CaCl 2 ·2H 2 O.................................................................................0.01g
Ferric ammonium citrate solution............................................20.0mL
Ferric Ammonium Citrate Solution:
Composition per 20.0mL:
Ferric ammonium citrate.............................................................0.05g
Preparation of Ferric Ammonium Citrate Solution: Add fer-
ric ammonium citrate to 20.0mL of distilled/deionized water. Filter
sterilize.
Preparation of Medium: Add components, except ferric ammoni-
um citrate solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add the
sterile ferric ammonium citrate solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes. Incubate inoculated
medium at 30°C under 60% H 2 + 25% N 2 + 10% CO 2 + 5% O 2 .
Use:  For the cultivation and maintenance of Alcaligenes eutrophus,
Hydrogenophaga flava, and Hydrogenophaga pseudoflava.