Mineral Medium with 2-Hydroxybiphenyl (DSMZ Medium 465a)

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with 2-Hydroxybiphenyl
(DSMZ Medium 465a)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per1010.0mL:

Na 2 HPO 4 ·2H 2 O.............................................................................3.5g
KH 2 PO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.1g
Ca(NO 3 ) 2 ·4H 2 O...........................................................................0.05g
Hydroxybiphenyl solution .......................................................10.0mL
Trace elements solution SL-4 ....................................................1.0mL
pH 7.25 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6 ................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Hydroxybiphenyl Solution:
Composition per 100.0mL:
2-Hydroxybiphenyl.......................................................................5.0g
Preparation of Hydroxybiphenyl Solution: Add 2-hydroxybi-
phenyl to 100.0mL ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except hydroxybiphenyl
solution, to 1.0L distilled/deionized water. Adjust pH to 7.25. Autoclave
for 15 min at 15 psi pressure–121°C. Aseptically add hydroxybiphenyl
solution to a sterile culture vessel so that the final concentration of 2-hy-
droxybiphenyl will be 0.5g/L medium. Let the ethanol evaporate under
sterile conditions. Aseptically add the liquid medium to the culture ves-
sel to achieve the appropriate concentration of biphenyl.
Use: For the cultivation of Pseudomonas sp. and other hydroxybiphe-
nyl-utilizing bacteria.