Mineral Medium with 6-Methylnicotinate (DSMZ Medium 465h)

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with 6-Methylnicotinate
(DSMZ Medium 465h)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·2H 2 O.............................................................................3.5g
KH 2 PO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.1g
Ca(NO 3 ) 2 ·4H 2 O...........................................................................0.05g
Nicotinate solution...................................................................10.0mL
Selenite-tungstate solution.........................................................1.0mL
Vitamin solution.........................................................................1.0mL
Trace elements solution SL-4 ....................................................1.0mL
pH 7.25 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6 ................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Vitamin B 12 ..............................................................................50.0mg
Pantothenic acid.......................................................................50.0mg
Riboflavin ................................................................................50.0mg
Alpha-lipoic acid......................................................................50.0mg
p-Aminobenzoic acid...............................................................50.0mg
Thiamine-HCl·2H 2 O................................................................50.0mg
Nicotinic acid...........................................................................25.0mg
Nicotinamide............................................................................25.0mg
Biotin .......................................................................................20.0mg
Folic acid..................................................................................20.0mg

Pyridoxamine-HCl...................................................................10.0mg

Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Selenite-Tungstate Solution
Composition per liter:
NaOH............................................................................................0.5g
Na 2 WO 4 ·2H 2 O...........................................................................4.0mg
Na 2 SeO 3 ·5H 2 O...........................................................................3.0mg
Preparation of Selenite-Tungstate Solution: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sparge with 100% N 2 . Filter sterilize.
Nicotinate Solution:
Composition per 10.0mL:
6-Methylnicotinate........................................................................5.0g
Preparation of Nicotinate Solution: Add nicotinate to 10.0mL
distilled/deionized wate. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Cool to room temperature.
Preparation of Medium:  Add components, except nicotinate solu-
tion, selenite-tungstate solution, and vitamin solution, to 9898.0mL dis-
tilled/deionized water. Adjust pH to 7.25. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Aseptically add 10.0mL
nicotinate solution, 1.0mL selenite-tungstate solution, and 1.0mL vita-
min solution. Mix thoroughly. Aseptically distribute to sterile tubes or
flasks.
Use: For the cultivation of Paenibacillus sp. and other nicotinate-utiliz-
ing bacteria.