Mineral Medium (N 4 )

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium (N 4 )

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

HEPES buffer ........................................................................... 11.92g
NaCl..............................................................................................0.5g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
KH 2 PO 4 ........................................................................................0.2g
MgSO 4 ·7H 2 O..............................................................................0.04g
CaCl 2 ·2H 2 O................................................................................0.02g
FeSO 4 /EDTA solution.............................................................10.0mL
Phenol Red (0.04%)...................................................................1.0mL
FeSO 4 /EDTA Solution:
Composition per 100.0mL:
EDTA........................................................................................0.019g
FeSO 4 ·7H 2 O.............................................................................0.018g
Preparation of FeSO 4 /EDTA Solution:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Bacillus species.