Mineral Medium, Nagel and Andreesen (DSM Medium 461)

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium, Nagel and Andreesen
(DSM Medium 461)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O................................................................................0.5g
NH 4 Cl ...........................................................................................0.3g
NaCl............................................................................................0.05g
CaCl 2 ...........................................................................................0.01g
MnSO 4 ........................................................................................0.01g
Phosphate solution...................................................................50.0mL
Vitamin solution.........................................................................5.0mL
Trace elements solution SL-10 ..................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Phosphate Solution:
Composition per 50.0mL:
Na 2 HPO 4 ·2H 2 O...........................................................................1.45g
KH 2 PO 4 .......................................................................................0.25g
Preparation of Phosphate Solution : Add components to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.
Vitamin Solution:
Composition per liter:
Folic acid.....................................................................................20.0g
α-Lipoic acid............................................................................50.0mg
p-Aminobenzoic acid...............................................................50.0mg
Pantothenic acid.......................................................................50.0mg
Riboflavin ................................................................................50.0mg
Thamine·HCl............................................................................50.0mg
Vitamin B 12 ..............................................................................50.0mg
Nicotinamide............................................................................25.0mg
Biotin .......................................................................................20.0mg
Nicotinic acid...........................................................................20.0mg
Pyridoxamine·HCl ...................................................................10.0mg
Preparation of Vitamin Solution : Add components to distilled/
deionized water and bring volume to 1.0L Mix thoroughly. Stir for 2 hr.
Filter sterilize.
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except phosphate solu-
tion, vitamin solution, and trace elements solution SL-10, to distilled/
deionized water and bring volume to 944.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Aseptically add 50.0mL of
sterile phosphate solution, 5.0mL of sterile vitamin solution, and
1.0mL of sterile trace elements solution SL-10. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use: For the cultivation of Arthrobacter species, Mycobacterium spe-
cies, Paracoccus denitrificans, Pseudomonas putida, and Rhodococ-
cus species.