Mineral Medium with PCP (DSMZ Medium 465b)

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Mineral Medium with PCP
(DSMZ Medium 465b)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·2H 2 O.............................................................................3.5g
KH 2 PO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.1g
Ca(NO 3 ) 2 ·4H 2 O...........................................................................0.05g
Pentachlorophenol solution....................................................100.0mL
Trace elements solution SL-4 ....................................................1.0mL
pH 7.25 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6 ................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Pentachlorophenol Solution:
Composition per liter:
Pentachlorophenol ........................................................................1.0g
Preparation of Pentachlorophenol Solution: Add pentachloro-
phenol to 1.0L 0.1M NaOH. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except pentachlorophe-
nol solution, to 900.0mL distilled/deionized water. Adjust pH to 7.25.
Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add
100.0mL sterile pentachlorophenol solution. Mix thoroughly. Asepti-
cally distribute to sterile tubes or flasks.
Use: For the cultivation of Sphingobium chlorophenolicum=Sphingomo-
nas chlorophenolica and other pentachlorophenol-utilizing bacteria.