Minimal Broth, Davis

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Minimal Broth, Davis

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 .........................................................................................7.0g
KH 2 PO 4 .........................................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
Glucose .........................................................................................1.0g
Sodium citrate...............................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.1g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to cold distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation, cultivation, and characterization of nutritional
mutants of Escherichia coli. Also recommended for the isolation and
characterization of nutritional mutants from wild-type strains of Bacil-
lus subtilis when used in conjunction with minimal agar Davis and
antibiotic medium 3.