Minimal F-Top Agar

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Minimal F-Top Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................8.0g
Agar ..............................................................................................4.5g
K 2 HPO 4 .........................................................................................2.1g
KH 2 PO 4 .........................................................................................0.6g
(NH 4 ) 2 SO 4 .....................................................................................0.3g
Glucose .........................................................................................0.3g
Sodium citrate.............................................................................0.15g
MgSO 4 ·7H 2 O..............................................................................0.03g
pH 7.0 ± 0.2 at 25°C
Preparation of Minimal Agar: Add components to cold distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Gently
heat and bring to boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the distribution of bacteriophage or bacterial cells evenly in
a thin layer over the surface of a plate.