Minimal Medium for Denitrifying Bacteria

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Minimal Medium for
Denitrifying Bacteria

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................980.0mL
Solution B................................................................................10.0mL
Solution C................................................................................10.0mL
Solution A:
Composition per 980.0mL:
KNO 3 ............................................................................................5.0g
Carbon source...............................................................................4.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
K 2 HPO 4 ·3H 2 O ............................................................................0.87g
KH 2 PO 4 .......................................................................................0.54g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C.
Solution B:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................2.0g
Preparation of Solution B: Add MgSO 4 ·7H 2 O to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution C:
Composition per 100.0mL:
CaCl 2 ·2H 2 O ..................................................................................0.2g
FeSO 4 ·7H 2 O..................................................................................0.1g
MnSO 4 ·H 2 O................................................................................0.05g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 MoO 4 ·2H 2 O..........................................................................0.01g
HCl (0.1N solution) ...............................................................100.0mL
Preparation of Solution C: Combine components. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Aseptically combine 980.0mL of cooled
sterile solution A, 10.0mL of cooled sterile solution B, and 10.0mL of
cooled sterile solution C. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use: For the isolation and cultivation of denitrifying bacteria.