Mixotrophic Nitrobacter Medium (LMG Medium 246)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Mixotrophic Nitrobacter Medium
(LMG Medium 246)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaNO 2 ..........................................................................................2.0g
Yeast extract................................................................................1.50g
Peptone .......................................................................................1.50g
Na-pyruvate ................................................................................0.55g
Stock solution ........................................................................100.0mL
Trace elements solution.............................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Stock Solution:
Composition per liter:
NaCl..............................................................................................5.0g
KH 2 PO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCO 3 .........................................................................................0.07g
Preparation of Stock Solution: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
(NH 4 )Mo7O 2 ........................................................................437.10mg
FeSO 4 ·7H 2 O...........................................................................97.30mg
ZnSO 4 ·7H 2 O..........................................................................43.10mg
H 3 BO 3 ....................................................................................39.40mg
MnSO 4 ·H 2 O...........................................................................33.80mg
CuSO 2 ·5H 2 O..........................................................................25.00mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 with
NaOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the cultivation of mixotrophic Nitrobacter spp.