Mixotrophic Nitrobacter Medium, 10% (DSMZ Medium 756b)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Mixotrophic Nitrobacter Medium, 10%
(DSMZ Medium 756b)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaNO 2 ..........................................................................................2.0g
Yeast extract................................................................................0.15g
Peptone .......................................................................................0.15g
Na-pyruvate ..............................................................................0.055g
Stock solution ........................................................................100.0mL
Trace elements solution.............................................................1.0mL
pH 8.6 ± 0.2 at 25°C
Stock Solution:
Composition per liter:
NaCl..............................................................................................5.0g
KH 2 PO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCO 3 ........................................................................................0.07g
Preparation of Stock Solution: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
FeSO 4 ·7H 2 O.............................................................................97.3mg
H 3 BO 3 ......................................................................................49.4mg
ZnSO 4 ·7H 2 O............................................................................43.1mg
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O...............................................................37.1mg
MnSO 4 ·2H 2 O...........................................................................33.8mg
CuSO 4 ·5H 2 O............................................................................25.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.6. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation of Nitrobacter hamburgensis and Nitrobacter
vulgaris.