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MLCB Agar (Mannitol Lysine Crystal Violet-Brilliant Green Agar)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]
MLCB Agar
(Mannitol Lysine Crystal Violet-Brilliant Green Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .......................................................................................10.0g
Yeast extract..................................................................................5.0g
L- Lysine·HCl.................................................................................5.0g
NaCl..............................................................................................4.0g
Na 2 S 2 O 3 ........................................................................................4.0g
Mannitol........................................................................................3.0g
Beef extract...................................................................................2.0g
Ferric ammonium citrate...............................................................1.0g
Crystal Violet..............................................................................0.01g
Brilliant Green.........................................................................12.5mg
pH 6.8 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Do not autoclave. Cool to 50°C. Pour into
sterile Petri dishes in 20.0mL volumes.
Use: For the selective isolation and cultivation of Salmonella species
from fecal material and foods.
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