MM10 Agar (Modified Medium 10 Agar)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

MM10 Agar
(Modified Medium 10 Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Base........................................................................................954.0mL
Dithiothreitol solution..............................................................20.0mL
Sheep blood .............................................................................20.0mL
Na 2 CO 3  solution........................................................................5.0mL
Menadione solution ...................................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Base :
Composition per 954.0mL:
Agar............................................................................................15.0g
Casein peptone..............................................................................2.0g
Glucose.........................................................................................1.0g
Sodium formate ............................................................................1.0g
KNO 3 ............................................................................................0.5g
Yeast extract..................................................................................0.5g
Hemin..........................................................................................0.01g
Mineral salt solution 1 .............................................................38.0mL
Mineral salt solution 2 .............................................................38.0mL
Sodium lactate solution..............................................................4.0mL
Preparation of Base: Add components to distilled/deionized water
and bring volume to 954.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
Mineral Salt Solution 1:
Composition per 100.0mL:
K 2 HPO 4 .........................................................................................0.6g
Preparation of Mineral Salt Solution 1: Add K 2 HPO 4 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Mineral Salt Solution 2:
Composition per 100.0mL:
NaCl..............................................................................................1.2g
(NH 4 ) 2 SO 4 .....................................................................................1.2g
KH 2 PO 4 .........................................................................................0.6g
CaCl 2 ...........................................................................................0.12g
Preparation of Mineral Salt Solution 2: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Sodium Lactate Solution:
Composition per 100.0mL:
Sodium lactate.............................................................................60.0g
Preparation of Sodium Lactate Solution: Add sodium lactate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Dithiothreitol Solution:
Composition per 100.0mL:
Dithiothreitol.................................................................................1.0g
Preparation of Dithiothreitol Solution: Add dithiothreitol to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Menadione Solution:
Composition per 100.0mL:
Vitamin K 1 (phytomenadione)....................................................0.05g
Ethanol (95% solution)..........................................................100.0mL
Preparation of Menadione Solution: Add vitamin K 1 to
100.0mL of ethanol. Mix thoroughly. Filter sterilize.
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3  ........................................................................................ 8.0g
Preparation of Na 2 CO 3 Solution: Add Na 2 CO 3  to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: To 954.0mL of sterile cooled base, asep-
tically add 20.0mL of sterile dithiothreitol solution, 20.0mL of sterile,
defibrinated sheep blood, 5.0mL of sterile Na 2 CO 3  solution, and
1.0mL of sterile menadione solution. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile screw-capped tubes.
Use: For the isolation and quantitation of plaque bacteria, especially
Streptococcus mutans, Streptococcus sanguis, and Streptococcus sali-
varius.