MMA Salts Medium

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

MMA Salts Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar, noble..................................................................................20.0g
K 2 HPO 4 .........................................................................................1.2g
KH 2 PO 4 .......................................................................................0.62g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl..............................................................................................0.1g
CaCl 2 ·6H 2 O ................................................................................0.05g
ZnSO 4 ·7H 2 O.............................................................................70.0μg
H 3 BO 3 .......................................................................................10.0μg
MnSO 4 ·5H 2 O............................................................................10.0μg
Na 2 MoO 4 ·2H 2 O........................................................................10.0μg
CoCl 2 ·6H 2 O................................................................................5.0μg
CuSO 4 ·5H 2 O...............................................................................5.0μg
FeCl 3 ·6H 2 O................................................................................1.0mg
Monomethylamine solution.....................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Monomethylamine Solution:
Composition per 10.0mL:
Monomethylamine........................................................................1.0g
Preparation of Monomethylamine Solution:  Add monometh-
ylamine to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except monometh-
ylamine solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add
10.0mL of sterile methylamine solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Hyphomicrobium species and Methylophi-
lus methylotrophus.