MMB Medium

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

MMB Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.........................................................................................1.0g
(NH 4 ) 2 SO 4 ..................................................................................0.25g
Peptone .......................................................................................0.15g
Yeast extract................................................................................0.15g
Glucose solution......................................................................20.0mL
Hutner's basal salts solution.....................................................20.0mL
Vitamin solution.......................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Glucose Solution:
Composition per 20.0mL:
Glucose.........................................................................................1.5g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 20.0mL. Mix thoroughly. Filter steril-
ize.
Hutner’s Basal Salts Solution:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O ..............................................................................3.335g
FeSO 4 ·7H 2 O.............................................................................99.0mg
(NH 4 ) 6 MoO 7 O 24 ·4H 2 O............................................................9.25mg
"Metals 44"..............................................................................50.0mL
"Metals 44":
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.............................................................................1.095g
FeSO 4 ·7H 2 O..................................................................................0.5g
Sodium EDTA.............................................................................0.25g
MnSO 4 ·H2O..............................................................................0.154g
CuSO 4 ·5H 2 O............................................................................39.2mg
Co(NO 3 ) 2 ·6H 2 O .......................................................................24.8mg
Na 2 B 4 O 7 ·10H 2 O.......................................................................17.7mg
Preparation of “Metals 44”: Add sodium EDTA to distilled/de-
ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few
drops of concentrated H 2 SO 4 to retard precipitation of heavy metal
ions. Add remaining components. Mix thoroughly. Bring volume to
100.0mL with distilled/deionized water.
Preparation of Hutner’s Basal Salts Solution:  Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5
with KOH. Add remaining components. Add distilled/deionized water
to 1.0L. Adjust pH to 6.8.
Vitamin Solution:
Composition per 100.0mL:
Vitamin B 12 ..............................................................................0.01mg
Calcium  DL -pantothenate...........................................................0.5mg
Nicotinamide..............................................................................0.5mg
Pyridoxine·HCl..........................................................................0.5mg
Riboflavin ..................................................................................0.5mg
Thiamine·HCl ............................................................................0.5mg
Biotin .........................................................................................0.2mg
Folic acid....................................................................................0.2mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except glucose solu-
tion and vitamin solution, to distilled/deionized water and bring vol-
ume to 970.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 20.0mL of sterile glucose solution
and 10.0mL of sterile vitamin solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use:  For the cultivation of Angulomicrobium tetraedrale, Labrys
monachus, Prosthecomicrobium polyspheroidum, and Aquabacter
spiritensis.