MMJS Medium (Modified) (DSMZ Medium 1121)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

MMJS Medium (Modified)
(DSMZ Medium 1121)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ...........................................................................................20.0g
MgSO 4 ·7H 2 O................................................................................4.0g
Sulfur, elemental ..........................................................................3.0g
MgCl 2 ·6H 2 O..................................................................................3.0g
Na 2 S 2 O 3 ·5H 2 O..............................................................................2.0g
NH 4 Cl ........................................................................................1.25g
CaCl 2 ·2H 2 O...................................................................................0.8g
KCl .............................................................................................0.33g
K 2 HPO 4 .......................................................................................0.09g
KH 2 PO 4 .......................................................................................0.07g
Fe 2 (SO 4 ) 3 ·7H 2 O..........................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................1.0mg
H 2 WO 4 ......................................................................................1.0mg
NiCl 2 ·6H 2 O................................................................................1.0mg
Bicarbonate solution................................................................10.0mL
Trace elements solution ..........................................................10.0mL
Vitamin solution.........................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Nitrilotriacetic acid.......................................................................1.5g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O.................................................................................0.5g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
NaBr............................................................................................0.01g
SrCl 2 ·6H 2 O .................................................................................0.01g
KI................................................................................................0.01g
Na 2 MoO 4 ·4H 2 O.........................................................................1.0mg
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl........................................................................10.0mg
Thiamine-HCl·2H 2 O..................................................................5.0mg
Riboflavin..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
D -Ca-pantothenate .....................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid.................................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H 2 + 20% CO 2 . Filter sterilize.
Bicarbonate Solution :
Composition per 10.0mL:
NaHCO 3 ........................................................................................2.0g
Preparation of Bicarbonate Solution:  Add NaHCO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium:  Add components, except trace elements
solution, bicarbonate solution, and vitamin solution, to distilled/deion-
ized water and bring volume to 980.0mL. Mix thoroughly. Adjust the
pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature. Aseptically add the trace elements solution, bicarbonate
solution, and vitamin solution. Sparge wtih a gas mixture of 80% N 2 +
20% CO 2 for 5 min. Compress gas mixture of 79% N 2 + 20% CO 2 + 1%
O 2 into gas phase (> 80% volume of the tube or bottle) at 2 atm.
Use:  For the cultivation Sulfurivirga caldicuralii.