MMN Agar

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

MMN Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
D -Glucose....................................................................................10.0g
Malt extract...................................................................................3.0g
KH 2 PO 4 .........................................................................................0.5g
Ammonium tartrate.....................................................................0.25g
MgSO 4 ·7H 2 O..............................................................................0.15g
CaCl 2 ...........................................................................................0.05g
NaCl..........................................................................................0.025g
Thiamine·HCl ............................................................................0.1mg
FeCl 3 solution ............................................................................1.2mL
FeCl 3 Solution:
Composition per 100.0mL:
FeCl 3 .............................................................................................1.0g
Preparation of FeCl 3 Solution:  Add FeCl 3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Cenococcum geophilum,
Cortinarius species, Gyrodon lividus, Hebeloma crustuliniforme, Hebe-
loma pusillum, Hygrophorous purpurascens, Hygrophorus russula,
Laccaria bicolor, Laccaria laccata, Lyophyllum fumosum, Lyophyllum
shimeji, Macrolepiota rhacodes, Obolarina dryophila, Paxillus atro-
mentosus, Phaeolepiota aurea, Pisolithus tinctoruis, Rhizopogon colos-
sus, Rhizopogon ellenae, many Rhizopogon species, Sarcodon aspratu,
Scleroderma albidum, Scleroderma aurantium, many Suillus species,
Tricholoma flavovirens, and many Tricholoma species.