MMS Medium for Thermotoga neapolitana

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

MMS Medium for Thermotoga neapolitana

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................6.93g
Starch ............................................................................................5.0g
MgSO 4 ·7H 2 O..............................................................................1.75g
MgCl 2 ·6H 2 O................................................................................1.38g
KH 2 PO 4 .........................................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.5g
CaCl 2 ...........................................................................................0.38g
KCl..............................................................................................0.16g
NaBr.........................................................................................25.0mg
H 3 BO 3 ........................................................................................7.5mg
SrCl 2 ·6H 2 O.................................................................................3.8mg
(NH 4 ) 2 Ni(SO 4 ) 2 ..........................................................................2.0mg
Resazurin ...................................................................................1.0mg
KI...........................................................................................0.025mg
Wolfe’s mineral solution..........................................................15.0mL
pH 6.5 ± 0.2 at 25°C
Wolfe’s Mineral Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·H 2 O..................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.1g
CoCl 2 ·6H 2 O ..................................................................................0.1g
CaCl 2 .............................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.01g
AlK(SO 4 ) 2 ·12H 2 O.......................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.01g
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L.
Preparation of Medium: Prepare and dispense medium under 80%
N 2 and 20% CO 2 . Add components to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5 with H 2 SO 4 .
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation and maintenance of Thermotoga neapolitana.