MP Agar

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MP Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Sodium acetate..............................................................................0.1g
Basal medium ...............................................................................1.0L
Sodium sulfide solution.............................................................3.0mL
pH 7.0–7.5 at 25°C
Basal Medium:
Composition per liter:
CaSO 4 ·2H 2 O (saturated solution) ............................................20.0mL
NH 4 Cl (4% solution)..................................................................5.0mL
Trace elements solution .............................................................5.0mL
MgSO 4 ·7H 2 O (1% solution)......................................................1.0mL
K 2 HPO 4 (1% solution)...............................................................1.0mL
Preparation of Basal Medium: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
Ferrous EDTA solution............................................................20.0mL
ZnSO 4 ·7H 2 O (0.1% solution)..................................................10.0mL
MnSO 4 ·4H 2 O (0.02% solution)...............................................10.0mL
CuSO 4 ·5H 2 O (0.00005% solution)..........................................10.0mL
H 3 BO 3 (0.1% solution)............................................................10.0mL
Co(NO 3 ) 2 or CoCl 2 ·6H 2 O (0.01% solution)............................10.0mL
Na 2 MoO 4 ·2H 2 O (0.01% solution)...........................................10.0mL
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Ferrous EDTA Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................7.0g
EDTA............................................................................................2.0g
HCl, concentrated......................................................................1.0mL
Preparation of Ferrous EDTA Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Sodium Sulfide Solution:
Composition per 10.0mL:
Na 2 S·9H 2 0.....................................................................................1.0g
Preparation of Sodium Sulfide Solution: Add Na 2 S·9H 2 0 to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Prepare freshly.
Preparation of Medium: Add sodium acetate and agar to 1.0L of
basal medium. Mix thoroughly. Adjust pH to 7.0–7.5. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 3.0mL of sterile sodium sulfide solution
immediately prior to dispensing. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile screw-capped tubes.
Use: For the isolation and cultivation of Beggiatoa species and myx-
otrophic strains of Thiothrix species from water and environmental
sources.