MP 5 Medium (Mineral Pectin 5 Medium)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MP 5 Medium
(Mineral Pectin 5 Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar solution .........................................................................500.0mL
Basal medium........................................................................250.0mL
Mineral solution.....................................................................250.0mL
pH 5.0–6.0 at 25°C
Agar Solution:
Composition per 500.0mL:
Agar............................................................................................15.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Basal Medium:
Composition per 250.0mL:
Na 2 HPO 4 .......................................................................................6.0g

Pectin, citrus or apple ...................................................................5.0g

KH 2 PO 4 .........................................................................................4.0g
NH 4 SO 4 .........................................................................................2.0g
Yeast extract..................................................................................1.0g
Preparation of Basal Medium:  Add components to distilled/de-
ionized water and bring volume to 250.0mL. Mix thoroughly. Gently
heat and bring to boiling.
Mineral Solution:
Composition per 250.0mL:
FeSO 4 (0.1% solution)...............................................................1.0mL
MgSO 4 ·7H 2 O (20% solution) ....................................................1.0mL
CaCl 2 ·2H 2 O (0.1% solution)......................................................1.0mL
H 3 BO 3 (0.001% solution) ..........................................................1.0mL
MnSO 4 ·H 2 O (0.001% solution).................................................1.0mL
ZnSO 4 ·7H 2 O (0.007% solution..................................................1.0mL
CuSO 4 ·5H 2 O (0.005% solution)................................................1.0mL
MoO 3 (0.001% solution)............................................................1.0mL
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 250.0mL. Mix thoroughly.
Preparation of Medium: Combine 250.0mL of basal medium and
250.0mL of mineral solution. Mix thoroughly. Adjust pH to 5.0–6.0 with
1N HCl. Autoclave the basal medium-mineral solution and agar solution
separately for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asep-
tically combine the two sterile solutions. Mix thoroughly. Pour immedi-
ately into sterile Petri dishes to prevent hydrolysis of the agar.
Use: For the cultivation of microorganisms that produce polygalacta-
nase.