MP 7 Medium (Mineral Pectin 7 Medium)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MP 7 Medium
(Mineral Pectin 7 Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Basal medium ........................................................................500.0mL
Mineral solution.....................................................................500.0mL
pH 7.2 ± 0.2 at 25°C
Basal Medium:
Composition per 500.0mL:
Agar ............................................................................................15.0g
Na 2 HPO 4 .......................................................................................6.0g
Pectin (citrus or apple)..................................................................5.0g
KH 2 PO 4 .........................................................................................4.0g
NH 4 SO 4 .........................................................................................2.0g
Yeast extract..................................................................................1.0g
Preparation of Basal Medium:  Add components to distilled/de-
ionized water and bring volume to 500.0mL. Mix thoroughly. Gently
heat and bring to boiling.
Mineral Solution:
Composition per 500.0mL:
FeSO 4 (0.1% solution)...............................................................1.0mL
MgSO 4 ·7H 2 O (20% solution) ....................................................1.0mL
CaCl 2 ·2H 2 O (0.1% solution)......................................................1.0mL
H 3 BO 3 (0.001% solution) ..........................................................1.0mL
MnSO 4 ·H 2 O (0.001% solution).................................................1.0mL
ZnSO 4 ·7H 2 O (0.007% solution..................................................1.0mL
CuSO 4 ·5H 2 O (0.005% solution..................................................1.0mL
MoO 3 (0.001% solution)............................................................1.0mL
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 500.0mL. Mix thoroughly.
Preparation of Medium: Combine 500.0mL of basal medium and
500.0mL of mineral solution. Mix thoroughly. Adjust pH to 7.2. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Pour into
sterile Petri dishes.
Use: For the cultivation of microorganisms that produce pectate lyase.