MRS Broth with Cysteine

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Broth with Cysteine

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................18.5g
Pancreatic digest of gelatin.........................................................10.0g
Beef extract...................................................................................8.0g
Yeast extract..................................................................................4.0g
Sodium acetate..............................................................................3.0g
K 2 HPO 4 .........................................................................................2.0g
Ammonium citrate........................................................................2.0g
Polysorbate 80...............................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O..............................................................................0.05g
L -Cysteine solution....................................................................0.5mL
pH 6.2 ± 0.2 at 25°C
L -Cysteine Solution:
Composition per 10.0mL:
L -Cysteine.....................................................................................0.1g
Preparation of  L -Cysteine Solution: Add 0.1g of  L -cysteine to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except  L -cysteine solu-
tion, to distilled/deionized water and bring volume to 999.5mL. Mix
thoroughly. Adjust pH to 6.2. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 0.5mL of sterile  L -cysteine solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Leuconostoc mesenteroides.