MRS Broth with Ethanol (LMG Medium 240)

[所属分类:培养基配方] [发布时间:2021-6-8] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Broth with Ethanol
(LMG Medium 240)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Peptone .......................................................................................10.0g
Beef extract...................................................................................5.0g
Sodium acetate·3H 2 O....................................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Diammonium hydrogen citrate.....................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O...........................................................................38.0mg
Ethanol...................................................................................100.0mL
Tween™ 80................................................................................1.0mL
pH 5.0± 0.2 at 25°C
Preparation of Medium: Add components, except ethanol, to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti-
cally add 100.0mL of sterile ethanol. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or sterile flasks.
Use: For the cultivation of Lactobacillus acetotolerans.