MS Medium (DSMZ Medium 670)

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MS Medium
(DSMZ Medium 670)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur............................................................................................5.0g
(NH 4 ) 2 SO 4 ....................................................................................2.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
K 2 HPO 4 .........................................................................................0.1g
KCl................................................................................................0.1g
Yeast extract solution...............................................................10.0mL
pH 3.5 ± 0.2 at 25°C
Yeast Extract Solution :
Composition per 10.0mL:
Yeast extract..................................................................................0.2g
Preparation of Yeast Extract Solution : Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Preparation of Medium: Sulfur is sterilized by steaming for 3
hours on each of 3 successive days. Add components, except sulfur and
yeast extract solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Adjust pH to 3.5 with 1N sulfuric acid. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically
add 5.0g sterile sulfur and 10.0mL sterile yeast extract solution. Mix
thoroughly. Distribute into tubes or flasks.
Use: For the cultivation and maintenance of Acidithiobacillus caldus
DSM9466.