MS Medium (DSMZ Medium 670)

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MS Medium
(DSMZ Medium 670)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 ....................................................................................2.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
K 2 HPO 4 .........................................................................................0.1g
KCl................................................................................................0.1g
Glucose solution ......................................................................20.0mL
Yeast extract solution...............................................................10.0mL
pH 3.0 ± 0.2 at 25°C
Yeast Extract Solution :
Composition per 10.0mL:
Yeast extract..................................................................................0.1g
Preparation of Yeast Extract Solution: Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Glucose Solution :
Composition per 20.0mL:
Glucose.........................................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 20.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except yeast extract so-
lution and glucose solution, to distilled/deionized water and bring vol-
ume to 970.0mL. Mix thoroughly. Adjust pH to 3.0 with 2N sulfuric
acid. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically add 20.0mL sterile glucose solution and 10.0mL sterile
yeast extract solution. Mix thoroughly. Distribute into tubes or flasks.
Use: For the cultivation and maintenance of Acidiphillum cryptum
DSM9467.