MS Medium (DSMZ Medium 670)

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MS Medium
(DSMZ Medium 670)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 .....................................................................................2.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
K 2 HPO 4 .........................................................................................0.1g
KCl................................................................................................0.1g
FeSO 4 solution.........................................................................50.0mL
pH 1.6 ± 0.2 at 25°C
FeSO 4 Solution :
Composition per 100.0mL:
FeSO 4 ·7H 2 O................................................................................40.0g
Preparation of FeSO 4 Solution : Add FeSO 4 ·7H 2 O to 0.2N sulfuric
acid and bring volume to 100.0mL. Mix thoroughly. Autoclave under
100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room tempera-
ture.
Preparation of Medium: Add components, except FeSO 4  solution,
to distilled/deionized water and bring volume to 950.0mL. Mix thor-
oughly. Adjust pH to 1.6 with 4N sulfuric acid. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 50.0mL sterile
FeSO 4  solution. Mix thoroughly. Distribute into tubes or flasks.
Use: For the cultivation and maintenance of Leptospirillum sp.
DSM9468.