MS Medium for Acidiphilum cryptum

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MS Medium for Acidiphilum cryptum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 .....................................................................................2.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
K 2 HPO 4 .........................................................................................0.1g
KCl................................................................................................0.1g
Glucose solution ......................................................................20.0mL
Yeast extract solution...............................................................10.0mL
pH 3.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
D -Glucose....................................................................................10.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................................1.0g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except glucose solu-
tion and yeast extract solution, to distilled/deionized water and bring
volume to 970.0mL. Mix thoroughly. Adjust pH to 3.0 with 2N H 2 SO 4 .
Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add
20.0mL of sterile glucose solution and 10.0mL of sterile yeast extract
solution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use:  For the cultivation of Acidiphilium cryptum.