MS Medium (Modified) (DSMZ Medium 1145)

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MS Medium (Modified)
(DSMZ Medium 1145)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O................................................................................7.0g
Sulfur, elemental ..........................................................................5.0g
NaS 2 O 3 ·5H 2 O ...............................................................................2.0g
MgCl 2 ·6H 2 O .................................................................................0.8g
KCl .............................................................................................0.48g
CaCl 2 ·2H 2 O ..................................................................................0.4g
MS Buffer..............................................................................200.0mL
Solution A................................................................................20.0mL
Solution D................................................................................10.0mL
Solution B..................................................................................1.5mL
pH 6.6 ± 0.2 at 25°C
MS Buffer Solution:
Composition per liter:
NaOH............................................................................................4.0g
Preparation of MS Buffer Solution:  Prepare anaerobic water by
sparging with double distilled water with constant gassing with 100%
N 2 . Add NaOH to double distilled/deionized anaerobic water and bring
volume to 1.0L. Mix thoroughly. Sparge with CO 2 to saturate. Filter
sterilize.
Solution A:
Composition per liter:
NH 4 Cl ......................................................................................100.0g
MgCl 2 ·6H 2 O .............................................................................100.0g
CaCl 2 ·2H 2 O ................................................................................40.0g
Preparation of Solution A:  Prepare anaerobic water by sparging
with double distilled water with constant gassing with 100% N 2 . Add
components to double distilled/deionized anaerobic water and bring
volume to 1.0L. Mix thoroughly. Adjust pH 4.0 with HCl.
Solution B:
Composition per liter:
K 2 HPO 4 ·3H 2 O ..........................................................................200.0g
Preparation of Solution B:  Prepare anaerobic water by sparging
with double distilled water with constant gassing with 100% N 2 . Add
K 2 HPO 4 ·3H 2 O to double distilled/deionized anaerobic water and bring
volume to 1.0L. Mix thoroughly.
Solution D:
Composition per liter:
Na 2 -EDTA.....................................................................................0.5g
CoCl 2 ·6H 2 O...........................................................................150.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
FeSO 4 ·7H 2 O...........................................................................100.0mg
ZnCL 2 ....................................................................................100.0mg
AlCl 3 ·6H 2 O..............................................................................40.0mg
Na 2 WO 4 ·2H 2 O.........................................................................30.0mg
CuCl 2 ·2H 2 O.............................................................................20.0mg
NiSO 4 ·6H 2 O.............................................................................20.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................10.0mg
H 2 SeO 3 .....................................................................................10.0mg
H 3 BO 3 ......................................................................................10.0mg
Preparation of Solution D:  Prepare anaerobic water by sparging
with double distilled water with constant gassing with 100% N 2 . Add
components to double distilled/deionized anaerobic water and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 3.0.
Preparation of Medium: Prepare anaerobic water by sparging with
double distilled water with constant gassing with 100% N 2 . Add com-
ponents, except sulfur, to double distilled/deionized anaerobic water
and bring volume to 1.0L. Adjust pH to 6.6. Autoclave for 15 min at
15 psi pressure–121°C. Sterilize sulfur separately in screw-capped
tubes by steaming in a water bath for 3 hr on each of 3 successive days.
Aseptically add the sterilized sulfur to the medium. Mix thoroughly.
Use: For the cultivation of Sulfurihydrogenibium kristjanssonii.