MS Medium for Thiobacillus caldus

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MS Medium for Thiobacillus caldus

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur, powdered...........................................................................5.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
K 2 HPO 4 .........................................................................................0.1g
KCl................................................................................................0.1g
Yeast extract solution...............................................................10.0mL
pH 3.5 ± 0.2 at 25°C
Preparation of Sulfur:  Sterilize powdered elemental sulfur by
steaming for 3 hr at 0 psi pressure–100°C on 3 successive days.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................................0.2g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except elemental sulfur
and yeast extract solution, to distilled/deionized water and bring volume
to 990.0mL. Mix thoroughly. Adjust pH to 3.5 with 1N sulfuric acid. Au-
toclave for 15 min at 15 psi pressure–121°C. Aseptically add 5.0g of
sterile elemental sulfur and 10.0mL of sterile yeast extract solution. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Thiobacillus caldus.