MS Medium for Thiobacillus thiooxidans

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MS Medium for Thiobacillus thiooxidans

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur, powdered...........................................................................5.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
K 2 HPO 4 .........................................................................................0.1g
KCl................................................................................................0.1g
pH 3.5 ± 0.2 at 25°C
Preparation of Sulfur:  Sterilize powdered elemental sulfur by
steaming for 3 hr at 0 psi pressure–100°C on three successive days.
Preparation of Medium: Add components, except elemental sul-
fur, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 3.5 with 1N sulfuric acid. Autoclave for 15 min
at 15 psi pressure–121°C. Aseptically add 5.0g of sterile elemental sul-
fur. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Thiobacillus thiooxidans.