MSA-Fe Medium

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

MSA-Fe Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................5.8g
Pancreatic digest of casein............................................................2.0g
Yeast extract..................................................................................2.0g
MgCl 2 ·6H 2 O .................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
Mercaptoethanesulfonic acid........................................................0.5g
K 2 HPO 4 ·3H 2 O ..............................................................................0.4g
Resazurin...................................................................................0.5mg
Trace elements solution...........................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Trace Elements Solution:
Composition per 100.0mL:
Disodium EDTA·2H 2 O............................................................50.0mg
CoCl 2 ·6H 2 O.............................................................................15.0mg
FeSO 4 ·7H 2 O.............................................................................10.0mg
MnCl 2 ·4H 2 O ............................................................................10.0mg
ZnCl 2 ........................................................................................10.0mg
AlCl 3 ·6H 2 O................................................................................4.0mg
Na 2 WO 4 ·2H 2 O...........................................................................3.0mg
CuCl 2 ·2H 2 O...............................................................................2.0mg
NiSO 4 ·6H 2 O...............................................................................2.0mg
H 2 SeO 3 .......................................................................................1.0mg
H 3 BO 3 ........................................................................................1.0mg
Na 2 MoO 4 ·2H 2 O.........................................................................1.0mg
Preparation of Trace Elements Solution : Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium:  Prepare and dispense medium under
100% N 2 . Add components to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Adjust pH to 7.5 with 2N NaOH. Sparge
with 100% N 2 for 30 min. Anaerobically distribute into tubes. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Bacillus infermus.