M-Slanetz Enterococcus Broth Base with Triphenyltetrazolium Chloride

[所属分类:培养基配方] [发布时间:2021-6-9] [发布人:网站管理员2] [阅读次数:] [返回]

M-Slanetz Enterococcus Broth Base
with Triphenyltetrazolium Chloride

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose......................................................................................100.0g
Casein enzymic hydrolysate.......................................................25.0g
Peptic digest of animal tissue .....................................................15.0g
Yeast extract................................................................................10.0g
K 2 HPO 4 .........................................................................................4.0g
Glucose .........................................................................................2.0g
NaN 3 .............................................................................................0.4g
Triphenyltetrazolium chloride solution......................................5.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without triphenyltetrazolium chloride solution,
is available as a premixed powder from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Triphenyltetrazolium Choride Solution:
Composition per 5.0mL:
Triphenyltetrazolium chloride.......................................................0.1g
Preparation of Triphenyltetrazolium Choride Solution: Add
triphenyltetrazolium chloride to distilled/deionized water and bring
volume to 5.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except triphenyltetrazo-
lium chloride solution, to distilled/deionized water and bring volume to
1.0L.Mix thoroughly. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 5.0mL triphenyltetrazolium chloride so-
lution. Mix thoroughly.
Use: For the isolation, cultivation, and enumeration of entercocci in
water, sewage, and feces by the membrane filter method. For the direct
plating of specimens for the detection and enumeration of fecal strep-
tococci. For the isolation and detection of enterococci using the mem-
brane filter technique.