MSVP Agar

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

MSVP Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 984.0mL:

Agar, noble..................................................................................15.0g
HEPES (N-[2-hydroxyethyl]piperazine-
N′-2-ethanesulfonic acid) buffer........................................2.383g
(NH 4 ) 2 SO 4 ..................................................................................0.24g
CaCl 2 ·2H 2 O ................................................................................0.06g
MgSO 4 ·7H 2 O..............................................................................0.06g
Na 2 HPO 4 .....................................................................................0.03g
KH 2 PO 4 .......................................................................................0.02g
FeSO 4  (10mM solution).............................................................1.0mL
Sodium pyruvate solution..........................................................5.0mL
Vitamin solution.........................................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Sodium Pyruvate Solution:
Composition per 50.0mL:
Sodium pyruvate.........................................................................10.0g
Preparation of Sodium Pyruvate Solution:  Add sodium pyru-
vate to distilled/deionized water and bring volume to 50.0mL. Mix
thoroughly. Filter sterilize.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl......................................................................100.0mg
p-Aminobenzoic acid...............................................................50.0mg
D -(+)-Calcium pantothenate ....................................................50.0mg
Nicotinic acid...........................................................................50.0mg
Riboflavin................................................................................50.0mg
Thiamine·HCl ..........................................................................50.0mg
Biotin.......................................................................................20.0mg
Folic acid .................................................................................20.0mg
Vitamin B 12 ................................................................................1.0mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except sodium pyru-
vate solution and vitamin solution, to distilled/deionized water and
bring volume to 994.0mL. Mix thoroughly. Gently heat and bring to
boiling. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add 5.0mL of sterile sodium
pyruvate solution and 1.0mL of sterile vitamin solution. Mix thorough-
ly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Leptothrix discophora.