M-Tetrathionate HiVeg Broth Base with Iodine

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

M-Tetrathionate HiVeg Broth Base with Iodine

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 S 2 O 3 ......................................................................................30.0g
Plant peptone No. 3.......................................................................5.0g
Synthetic detergent........................................................................1.0g
Iodine solution .........................................................................20.0mL
Source: This medium, without iodine solution, is available as a pre-
mixed powder from HiMedia.
Iodine Solution:
Composition per 20.0mL:
Iodine............................................................................................6.0g
KI..................................................................................................5.0g
Preparation of Iodine Solution:  Add iodine and KI to distilled/
deionized water and bring volume to 20.0mL. Mix thoroughly.
Preparation of Medium: Add components, except iodine solution,
to distilled/deionized water and bring volume to 980.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Do not autoclave. Cool to
40°C. Add 20.0mL of iodine solution. Mix thoroughly. Distribute into
tubes in 10.0mL volumes. Use medium the same day it is prepared.
Use: For the selective isolation and enrichment of Salmonella typhi
and other salmonellae from fecal specimens, sewage, and other speci-
mens.