Mueller-Hinton Agar with Horse Blood (LMG Medium 49)

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

Mueller-Hinton Agar with Horse Blood
(LMG Medium 49)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef, infusion from...................................................................300.0g
Acid hydrolysate of casein..........................................................17.5g
Agar............................................................................................17.0g
Starch............................................................................................1.5g
Horse blood, sterile defibrinated..............................................50.0mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium without horse blood is available as a premixed
powder from BD Diagnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components, except horse blood, to
950.0mL distilled/deionized water. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 50.0mL sterile horse blood. Mix thorough-
ly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Campylobacter spp.,
Arcobacter spp., Helicobacter spp., Moraxella lincolnii, and other bac-
teria.