Mueller-Hinton Agar with IsoVitaleX ® and Hemoglobin

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

Mueller-Hinton Agar
with IsoVitaleX ® and Hemoglobin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Component A.........................................................................490.0mL
Component B.........................................................................490.0mL
IsoVitaleX ® enrichment...........................................................20.0mL
pH 6.9 ± 0.2 at 25°C
Component A:
Composition per 490.0mL:
Beef infusion.............................................................................300.0g
Acid hydrolysate of casein..........................................................17.5g
Agar ............................................................................................17.0g
Starch ............................................................................................1.5g
Preparation of Component A: Add components to distilled/de-
ionized water and bring to 490.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Component B:
Composition per 490.0mL:
Hemoglobin ................................................................................10.0g
Preparation of Component B: Add hemoglobin to distilled/deion-
ized water and bring to 490.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
IsoVitaleX ® Enrichment:
Composition per liter:
Glucose .....................................................................................100.0g
L- Cysteine·HCl............................................................................25.9g
L- Glutamine.................................................................................10.0g
L- Cystine .......................................................................................1.1g
Adenine.........................................................................................1.0g
Nicotinamide adenine dinucleotide ............................................0.25g
Vitamin B 12 ...................................................................................0.1g
Thiamine pyrophosphate...............................................................0.1g
Guanine·HCl...............................................................................0.03g
Fe(NO 3 ) 3 ·6H 2 O...........................................................................0.02g
p-Aminobenzoic acid................................................................0.013g
Thiamine·HCl ............................................................................3.0mg
Source: The supplement solution IsoVitaleX ® enrichment is available
from BD Diagnostic Systems. This enrichment may be replaced by
supplement VX from BD Diagnostic Systems.
Preparation of IsoVitaleX ® Enrichment: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize. Warm to 45°–50°C.
Preparation of Medium: Aseptically combine 490.0mL of compo-
nent A, 490.0mL of component B, and 20.0mL of IsoVitaleX ® enrich-
ment. Mix thoroughly. Adjust pH to 6.9. Pour into sterile Petri dishes
in 20.0mL volumes.
Use: For the isolation and cultivation of Legionella pneumophila.