Mueller-Hinton Chocolate Agar

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

Mueller-Hinton Chocolate Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef infusion.............................................................................300.0g
Acid hydrolysate of casein..........................................................17.5g
Agar............................................................................................17.0g
Starch............................................................................................1.5g
Sheep blood .............................................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep
blood. Mix thoroughly. Gently heat to 70°C for 10 min. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Neisseria gonorrhoeae and
Neisseria meningitidis. For antimicrobial susceptibility testing of fastid-
ious microorganisms.