Mueller-Hinton II Agar

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

Mueller-Hinton II Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Acid hydrolysate of casein..........................................................17.5g
Agar ............................................................................................17.0g
Beef extract...................................................................................2.0g
Starch ............................................................................................1.5g
pH 7.3 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For antimicrobial disc diffusion susceptibility testing by the
Bauer-Kirby method of a variety of bacteria. This medium supple-
mented with 5% sheep blood is recommended for use in antimicrobial
susceptibility testing of Streptococcus pneumoniae and Haemophilus
influenzae.