Mueller-Tellurite Medium

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

Mueller-Tellurite Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casamino acids...........................................................................20.0g
Agar............................................................................................20.0g
Casein ...........................................................................................5.0g
KH 2 PO 4 .........................................................................................0.3g
MgSO 4 ·7H 2 O................................................................................0.1g
L- Tryptophan...............................................................................0.05g
Mueller-tellurite serum............................................................25.0mL
pH 7.4 ± 0.1 at 25°C
Mueller-Tellurite Serum:
Composition per 100.0mL:
K 2 TeO 3 solution............................................................................0.4g
Calcium pantothenate ................................................................0.2mg
Horse or beef serum, sterile.....................................................50.0mL
Sodium lactate solution............................................................40.0mL
Ethyl alcohol............................................................................10.0mL
Preparation of Mueller-Tellurite Serum: Add calcium pantoth-
enate to 1.0mL of distilled/deionized water. Autoclave for 15 min at 15
psi pressure–121°C. Add K 2 TeO 3 to 1.0mL of sterile distilled/deion-
ized water. To 40.0mL of cooled, sterile sodium lactate solution, add
filter-sterilized ethanol, sterile calcium pantothenate solution, sterile
serum, and K 2 TeO 3 solution. Mix thoroughly.
Sodium Lactate Solution:
Composition per 100.0mL:
Lactic acid (85% solution).......................................................50.0mL
Phenol Red solution (0.2g in 50% ethanol)...............................0.1mL
Preparation of Sodium Lactate Solution: Add lactic acid to dis-
tilled/deionized water and bring volume to 100.0mL. Add 0.1mL of
Phenol Red solution. Add enough 40% NaOH solution to adjust pH to
7.0. Gently heat and bring to boiling for 5 min. Add more NaOH solu-
tion to retain red color, if necessary. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 975.0mL. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool quickly to 50°C.
Aseptically add 25.0mL of Mueller-Tellurite serum. Mix thoroughly.
Distribute into sterile Petri dishes. Allow the surface of the plates to dry
by partially removing the covers during solidification.
Use: For the isolation, cultivation, and differentiation of Corynebacte-
rium diphtheriae.