MUG Bromcresol Purple Broth with Lactose

[所属分类:培养基配方] [发布时间:2021-6-10] [发布人:网站管理员2] [阅读次数:] [返回]

MUG Bromcresol Purple Broth with Lactose

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casein enzymic hydrolysate.......................................................17.0g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
Tryptophan....................................................................................1.0g
Bromcresol Purple......................................................................0.02g
4-Methylumbelliferyl-β- D -glucuronide (MUG).........................0.01g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes with inverted Durham tubes. Autoclave
for 20 min at 10 psi pressure–115°C.
Use: For the identification of Escherichia coli and coliform bacteria
from water samples by a fluorogenic assay method.